COURSE Introduction Choice of DNA: genomic DNA or cDNA Insertion of the chosen DNA into a vector Creation of a DNA library Library screening Creation of an expression library - - - - - - - - - - - -

Introduction If, as a Veterinary Scientist, you wish to determine the molecular origin of a fatal disease afflicting Arctic lemmings, or contribute to the body of knowledge within the cow, dog, mouse or pig genome projects, or even study the underlying cause as to why phenotypic determination in the ladybird can sometimes cause it to masquerade as a Colorado beetle (sic), then you will need to embark on molecular cloning. The discovery of restriction enzymes and reverse transcriptase, together with the engineering of cloning vectors, has brought about advances in recombinant DNA technology facilitating molecular cloning. The following basic steps are involved in the molecular cloning of genes. 1. Choice of DNA: genomic DNA or cDNA 2. Insertion of the chosen DNA into a vector 3. Creation of a DNA library 4. Library screening 5. Creation of an expression library

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